vsmc cell line a10 Search Results


rat a10 vsmcs  (ATCC)
96
ATCC rat a10 vsmcs
Rat A10 Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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a10 cell line  (LGC Standards)
91
LGC Standards a10 cell line
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
A10 Cell Line, supplied by LGC Standards, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/PCB+iso-Octane/pmc09928102-42-1-7
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vsmcs  (ATCC)
97
ATCC vsmcs
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Vsmcs, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/A-10/pm35285274-58-0-3
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rat myeloma cell line yb2 0  (ATCC)
96
ATCC rat myeloma cell line yb2 0
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Rat Myeloma Cell Line Yb2 0, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cd38  (BPS Bioscience)
93
BPS Bioscience cd38
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Cd38, supplied by BPS Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/TetR+HEK293+Recombinant+Cell+Line/us11535621-1942-7-8
Average 93 stars, based on 1 article reviews
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s cells  (AutoMate Scientific Inc)
95
AutoMate Scientific Inc s cells
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
S Cells, supplied by AutoMate Scientific Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture rat aortic smooth muscle cells a10  (ATCC)
95
ATCC cell culture rat aortic smooth muscle cells a10
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Cell Culture Rat Aortic Smooth Muscle Cells A10, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cell culture rat aortic smooth muscle cells a10 - by Bioz Stars, 2026-10
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lentiviral transduction 293t cells  (Addgene inc)
92
Addgene inc lentiviral transduction 293t cells
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Lentiviral Transduction 293t Cells, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentiviral transduction 293t cells - by Bioz Stars, 2026-10
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sds page gel  (Bio-Rad)
98
Bio-Rad sds page gel
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Sds Page Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/SDS/ppr0229071-62-12-19
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u87mg ecacc no 89081402 u251mg ecacc no 89181493 ln229 atcc atcc crl  (ATCC)
99
ATCC u87mg ecacc no 89081402 u251mg ecacc no 89181493 ln229 atcc atcc crl
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
U87mg Ecacc No 89081402 U251mg Ecacc No 89181493 Ln229 Atcc Atcc Crl, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/U-87+MG/pm35417708-427-183-192
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irradiated eg7 ova cells  (ATCC)
97
ATCC irradiated eg7 ova cells
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Irradiated Eg7 Ova Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/EL4/us07842289-2392-17-28
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hek 293t cells  (ATCC)
99
ATCC hek 293t cells
Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) <t>A10</t> cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).
Hek 293t Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/vsmc+cell+line+a10/293T/pm42127892-314-11-13
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Image Search Results


Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) A10 cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).

Journal: PLOS ONE

Article Title: Cultured rat aortic vascular smooth muscle cells do not express a functional TRPV1

doi: 10.1371/journal.pone.0281191

Figure Lengend Snippet: Western blot detections of TRPV1 protein in undifferentiated (-) and differentiated (diff) PC-12 cells (A), wild-type (WT) and TRPV1 KO (G12, H3) A10 cells (A and B), in growing (gr) and quiescent (quiesc) rat primary VSMCs (A and B), and in TRPV1 transfected HEK293T cells (B) using two different antibodies against TRPV1: mouse monoclonal antibody (mAb) against the C-terminus (A, upper panel) and rabbit polyclonal antibody (pAb) against N-terminus (A, lower panel and B), α/β-tubulin was used as a loading control. In each experiments cells of different passages were used. The membrane in experiment 3 was cut in the attempt to reduce unspecific binding by exposing only the part where TRPV1 was expected to bind the primary antibody. (b) One representative blot out of 4 independent western blot experiments with similar results is shown. (C) Capsaicin concentration-response curves measured by calcium microfluorimetry. The response to capsaicin was quantified by the area under the curve (AUC) and fitted by a sigmoidal function. HEK293T cells transfected with human TRPV1 (red) respond to capsaicin, in contrast to untransfected HEK293T cells (black).

Article Snippet: The A10 cell line was purchased from LGC Standards (Teddington, UK).

Techniques: Western Blot, Transfection, Control, Membrane, Binding Assay, Concentration Assay

TRPV1, GAPDH and 18S mRNA expression levels in growing and quiescent VSMCs (A), and in A10 cell line (B) determined by RT qPCR; (A and B) the graphs show Cp values from each independent biological experiment. DRG was used as a positive control. (C) Relative TRPV1 mRNA expression in CRISPR/Cas-edited A10 cells, compared to their normal counterpart; graph shows mean ± SD in the form of percentage relative to the amount of TRPV1 transcript in normal A10 cells of 4 independent biological experiments, each performed in triplicate (** P < 0.01; one-way ANOVA, Tukey post-hoc test). GAPDH was used as a housekeeping gene control.

Journal: PLOS ONE

Article Title: Cultured rat aortic vascular smooth muscle cells do not express a functional TRPV1

doi: 10.1371/journal.pone.0281191

Figure Lengend Snippet: TRPV1, GAPDH and 18S mRNA expression levels in growing and quiescent VSMCs (A), and in A10 cell line (B) determined by RT qPCR; (A and B) the graphs show Cp values from each independent biological experiment. DRG was used as a positive control. (C) Relative TRPV1 mRNA expression in CRISPR/Cas-edited A10 cells, compared to their normal counterpart; graph shows mean ± SD in the form of percentage relative to the amount of TRPV1 transcript in normal A10 cells of 4 independent biological experiments, each performed in triplicate (** P < 0.01; one-way ANOVA, Tukey post-hoc test). GAPDH was used as a housekeeping gene control.

Article Snippet: The A10 cell line was purchased from LGC Standards (Teddington, UK).

Techniques: Expressing, Quantitative RT-PCR, Positive Control, CRISPR, Control